Natural Variation at the FRD3 MATE Transporter Locus Reveals Cross-Talk between Fe Homeostasis and Zn Tolerance in Arabidopsis thaliana

Abstract : Zinc (Zn) is essential for the optimal growth of plants but is toxic if present in excess, so Zn homeostasis needs to be finely tuned. Understanding Zn homeostasis mechanisms in plants will help in the development of innovative approaches for the phytoremediation of Zn-contaminated sites. In this study, Zn tolerance quantitative trait loci (QTL) were identified by analyzing differences in the Bay-0 and Shahdara accessions of Arabidopsis thaliana. Fine-scale mapping showed that a variant of the Fe homeostasis-related FERRIC REDUCTASE DEFECTIVE3 (FRD3) gene, which encodes a multidrug and toxin efflux (MATE) transporter, is responsible for reduced Zn tolerance in A. thaliana. Allelic variation in FRD3 revealed which amino acids are necessary for FRD3 function. In addition, the results of allele-specific expression assays in F1 individuals provide evidence for the existence of at least one putative metal-responsive cis-regulatory element. Our results suggest that FRD3 works as a multimer and is involved in loading Zn into xylem. Cross-homeostasis between Fe and Zn therefore appears to be important for Zn tolerance in A. thaliana with FRD3 acting as an essential regulator.
Complete list of metadatas

Cited literature [41 references]  Display  Hide  Download

https://hal-montpellier-supagro.archives-ouvertes.fr/hal-02024149
Contributor : Pierre Berthomieu <>
Submitted on : Monday, February 18, 2019 - 10:35:28 PM
Last modification on : Sunday, October 6, 2019 - 2:54:01 PM
Long-term archiving on : Sunday, May 19, 2019 - 6:04:59 PM

File

Pineau12 QTL FRD3 tolérance ...
Publisher files allowed on an open archive

Identifiers

Citation

Christophe Pineau, Stéphanie Loubet, Cécile Lefoulon, Claude Chalies, Cécile Fizames, et al.. Natural Variation at the FRD3 MATE Transporter Locus Reveals Cross-Talk between Fe Homeostasis and Zn Tolerance in Arabidopsis thaliana. PLoS Genetics, Public Library of Science, 2012, 8 (12), pp.e1003120. ⟨10.1371/journal.pgen.1003120⟩. ⟨hal-02024149⟩

Share

Metrics

Record views

64

Files downloads

10