Ecology of Endozoicomonadaceae in three coral genera across the Pacific Ocean

Corentin Hochart (1) , Lucas Paoli (2) , Hans-Joachim Ruscheweyh (3) , Guillem Salazar (3) , Emilie Boissin (4) , Sarah Romac (5, 6) , Julie Poulain (5, 7) , Guillaume Bourdin (8) , Guillaume Iwankow (4) , Clémentine Moulin (9) , Maren Ziegler (10) , Barbara Porro (11, 12) , Eric Armstrong (7, 5, 4) , Benjamin Hume (13) , Jean-Marc Aury (14, 5) , Claudia Pogoreutz (15) , David Paz-García , Maggy Nugues (16, 4) , Sylvain Agostini (17) , Bernard Banaigs , Emmanuel Boss (8) , Chris Bowler (5, 18) , Colomban de Vargas , Éric Douville (19, 20) , Michel Flores , Didier Forcioli , Paola Furla , Eric Gilson , Fabien Lombard (21) , Stéphane Pesant , Stéphanie Reynaud , Olivier Thomas , Romain Troublé , Patrick Wincker (7) , Didier Zoccola , Denis Allemand , Serge Planes , Rebecca Vega Thurber , Christian Voolstra , Shinichi Sunagawa , Pierre Galand (22)

1 UCA [2017-2020] - Université Clermont Auvergne [2017-2020]
2 Institute of Microbiology and Swiss Institute of Bioinformatics
3 D-BIOL - Department of Biology [ETH Zürich]
4 CRIOBE - Centre de recherches insulaires et observatoire de l'environnement
5 GOSEE - Global Oceans Systems Ecology & Evolution - Tara Oceans
6 SBR - Station biologique de Roscoff = Roscoff Marine Station
7 UMR 8030 - Génomique métabolique
8 University of Maine
9 Fondation Tara Ocean
10 JLU - Justus-Liebig-Universität Gießen = Justus Liebig University
11 UMR MARBEC - MARine Biodiversity Exploitation and Conservation
12 IRCAN - Institut de Recherche sur le Cancer et le Vieillissement
13 Universität Konstanz
14 LBGB - Laboratoire de Bioinformatique pour la Génomique et la Biodiversité
15 KAUST - King Abdullah University of Science and Technology [Saudi Arabia]
16 LabEX CORAIL - Laboratoire d'Excellence CORAIL
17 Shimoda Marine Research Center
18 IBENS - Institut de biologie de l'ENS Paris
19 LSCE - Laboratoire des Sciences du Climat et de l'Environnement [Gif-sur-Yvette]
20 GEOTRAC - Géochrononologie Traceurs Archéométrie
21 LOV - Laboratoire d'océanographie de Villefranche
22 LECOB - Laboratoire d'Ecogéochimie des environnements benthiques

Abstract Health and resilience of the coral holobiont depend on diverse bacterial communities often dominated by key marine symbionts of the Endozoicomonadaceae family. The factors controlling their distribution and their functional diversity remain, however, poorly known. Here, we study the ecology of Endozoicomonadaceae at an ocean basin-scale by sampling specimens from three coral genera ( Pocillopora , Porites , Millepora ) on 99 reefs from 32 islands across the Pacific Ocean. The analysis of 2447 metabarcoding and 270 metagenomic samples reveals that each coral genus harbored a distinct new species of Endozoicomonadaceae . These species are composed of nine lineages that have distinct biogeographic patterns. The most common one, found in Pocillopora , appears to be a globally distributed symbiont with distinct metabolic capabilities, including the synthesis of amino acids and vitamins not produced by the host. The other lineages are structured partly by the host genetic lineage in Pocillopora and mainly by the geographic location in Porites . Millepora is more rarely associated to Endozoicomonadaceae . Our results show that different coral genera exhibit distinct strategies of host- Endozoicomonadaceae associations that are defined at the bacteria lineage level.

Domains: Environmental Sciences

Corentin Hochart, Lucas Paoli, Hans-Joachim Ruscheweyh, Guillem Salazar, Emilie Boissin, et al.. Ecology of Endozoicomonadaceae in three coral genera across the Pacific Ocean. Nature Communications, 2023, 14 (1), pp.3037. ⟨10.1038/s41467-023-38502-9⟩. ⟨hal-04176293⟩