Convergence in light transmission properties of transparent wing areas in clearwing mimetic butterflies

Charline Pinna (1) , Maëlle Vilbert (2) , Stephan Borensztajn (3) , Willy Daney de Marcillac (4, 5) , Florence Piron-Prunier (1) , Aaron F Pomerantz (6) , Nipam Patel (7) , Serge Berthier (4, 5) , Christine Andraud (2) , Doris Gomez (8) , Marianne Elias (1)

1 ISYEB - Institut de Systématique, Evolution, Biodiversité
2 CRC - Centre de Recherche sur la Conservation
3 IPGP (UMR_7154) - Institut de Physique du Globe de Paris
4 INSP - Institut des Nanosciences de Paris
5 INSP-E4 - Nanostructures et optique
6 UC Berkeley - University of California [Berkeley]
7 Marine Biological Laboratory Massachussetts USA
8 CEFE - Centre d’Ecologie Fonctionnelle et Evolutive

Müllerian mimicry is a positive interspecific interaction, whereby co-occurring defended prey species share a common aposematic signal that advertises their defences to predators. In Lepidoptera, aposematic species typically harbour conspicuous opaque wing colour pattern, which have convergent optical properties, as perceived by predators. Surprisingly, some aposematic mimetic species have partially or totally transparent wings, which raises the question of whether optical properties of such transparent areas are also under selection for convergence. To answer this question and to investigate how transparency is achieved in the first place, we conducted a comparative study of optics and structures of transparent wings in neotropical mimetic clearwing Lepidoptera. We quantified transparency by spectrophotometry and characterised clearwing microstructures and nanostructures by microscopy imaging. We show that transparency is convergent among co-mimics in the eyes of predators, despite a large diversity of underlying micro- and nanostructures. Notably, we reveal that nanostructure density largely influences light transmission. While transparency is primarily produced by modification of microstructure features, nanostructures may provide a way to fine-tune the degree of transparency. This study calls for a change of paradigm in transparent mimetic lepidoptera: transparency not only enables camouflage but can also be part of aposematic signals.

Müllerian mimicry is a positive interspecific interaction, whereby co-occurring defended prey species share a common aposematic signal that advertises their defences to predators. In Lepidoptera, aposematic species typically harbour conspicuous opaque wing colour pattern, which have convergent optical properties, as perceived by predators. Surprisingly, some aposematic mimetic species have partially or totally transparent wings, which raises the question of whether optical properties of such transparent areas are also under selection for convergence. To answer this question and to investigate how transparency is achieved in the first place, we conducted a comparative study of optics and structures of transparent wings in neotropical mimetic clearwing Lepidoptera. We quantified transparency by spectrophotometry and characterised clearwing microstructures and nanostructures by microscopy imaging. We show that transparency is convergent among co-mimics in the eyes of predators, despite a large diversity of underlying micro-and nanostructures. Notably, we reveal that nanostructure density

Domaines: Sciences du Vivant [q-bio]

Charline Pinna, Maëlle Vilbert, Stephan Borensztajn, Willy Daney de Marcillac, Florence Piron-Prunier, et al.. Convergence in light transmission properties of transparent wing areas in clearwing mimetic butterflies. 2020. ⟨hal-03012864⟩